Review



dds coverslip  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
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    Structured Review

    OriGene dds coverslip
    Direct adhesion of proteins to <t>DDS</t> surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion <t>of</t> <t>EGFP</t> showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.
    Dds Coverslip, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dds+coverslip/pmc09238377-71-10-16?v=OriGene
    Average 94 stars, based on 26 article reviews
    dds coverslip - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Facile single-molecule pull-down assay for analysis of endogenous proteins"

    Article Title: Facile single-molecule pull-down assay for analysis of endogenous proteins

    Journal: Physical biology

    doi: 10.1088/1478-3975/ab0792

    Direct adhesion of proteins to DDS surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion of EGFP showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.
    Figure Legend Snippet: Direct adhesion of proteins to DDS surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion of EGFP showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.

    Techniques Used: Fluorescence

    Endogenous α-tubulin DDS SiMPull using mammalian cell lysates. (a) The average number of α-tubulin molecules per field-of-view for traditional DDS SiMPull, pre-incubation of the detection antibodies in solution, and nonspecific binding. Error bars show the standard deviation from the mean. Intensity distributions and representative images for (b) DDS sandwich SiMPull, (c) pre-incubation of 1°AB and F(ab)-A647, and (d) pre-incubation of lysate, 1°AB, and F(ab)-A647. Scale bars, 10 μm.
    Figure Legend Snippet: Endogenous α-tubulin DDS SiMPull using mammalian cell lysates. (a) The average number of α-tubulin molecules per field-of-view for traditional DDS SiMPull, pre-incubation of the detection antibodies in solution, and nonspecific binding. Error bars show the standard deviation from the mean. Intensity distributions and representative images for (b) DDS sandwich SiMPull, (c) pre-incubation of 1°AB and F(ab)-A647, and (d) pre-incubation of lysate, 1°AB, and F(ab)-A647. Scale bars, 10 μm.

    Techniques Used: Incubation, Binding Assay, Standard Deviation



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    Direct adhesion of proteins to <t>DDS</t> surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion <t>of</t> <t>EGFP</t> showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.
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    Direct adhesion of proteins to <t>DDS</t> surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion <t>of</t> <t>EGFP</t> showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.
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    Image Search Results


    Direct adhesion of proteins to DDS surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion of EGFP showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.

    Journal: Physical biology

    Article Title: Facile single-molecule pull-down assay for analysis of endogenous proteins

    doi: 10.1088/1478-3975/ab0792

    Figure Lengend Snippet: Direct adhesion of proteins to DDS surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion of EGFP showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.

    Article Snippet: For experiments where a protein was adsorbed directly onto the DDS coverslip, the protein (EGFP, TP790050, OriGene; tubulin-TMR, TL590M, Cytoskeleton; α-synuclein, S-1001-1, rPeptide) was diluted to the appropriate concentration in bPBS and incubated for 10 minutes.

    Techniques: Fluorescence

    Endogenous α-tubulin DDS SiMPull using mammalian cell lysates. (a) The average number of α-tubulin molecules per field-of-view for traditional DDS SiMPull, pre-incubation of the detection antibodies in solution, and nonspecific binding. Error bars show the standard deviation from the mean. Intensity distributions and representative images for (b) DDS sandwich SiMPull, (c) pre-incubation of 1°AB and F(ab)-A647, and (d) pre-incubation of lysate, 1°AB, and F(ab)-A647. Scale bars, 10 μm.

    Journal: Physical biology

    Article Title: Facile single-molecule pull-down assay for analysis of endogenous proteins

    doi: 10.1088/1478-3975/ab0792

    Figure Lengend Snippet: Endogenous α-tubulin DDS SiMPull using mammalian cell lysates. (a) The average number of α-tubulin molecules per field-of-view for traditional DDS SiMPull, pre-incubation of the detection antibodies in solution, and nonspecific binding. Error bars show the standard deviation from the mean. Intensity distributions and representative images for (b) DDS sandwich SiMPull, (c) pre-incubation of 1°AB and F(ab)-A647, and (d) pre-incubation of lysate, 1°AB, and F(ab)-A647. Scale bars, 10 μm.

    Article Snippet: For experiments where a protein was adsorbed directly onto the DDS coverslip, the protein (EGFP, TP790050, OriGene; tubulin-TMR, TL590M, Cytoskeleton; α-synuclein, S-1001-1, rPeptide) was diluted to the appropriate concentration in bPBS and incubated for 10 minutes.

    Techniques: Incubation, Binding Assay, Standard Deviation